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Invitrogen™ Dynabeads™ FlowComp™ Human CD4 Kit
Description
Includes
1mL Antibody, 3mL FlowComp; Dynabeads and 40mL FlowComp; Release Buffer
This gentle and easy-to-use kit allows you to isolate flow-compatible human CD4+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.
You can expect a purity level of >95% and up to 75% recovery of human CD4+ cells.
Advantages
• No risk of your results being affected by the isolation method
• Gentle tube-based method—no columns needed
• High purity and recovery
• Bead-free cells—compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay
The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferating and functional capacity. This kit avoids contamination of your isolated CD4+ cells with CD14+ monocytes. You will save time and money, and get increased confidence in your results.
Starting sample
MNC or whole blood
Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 mL whole blood.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Type | T Cells (CD4) |
| Content And Storage | The Kit Contains: • 1 mL Antibody • 3 mL FlowComp™ Dynabeads™ • 40 mL FlowComp™ Release Buffer. Store at: 2°C to 8°C. |
| Product Type | Superparamagnetic Beads |
| Isolation Technology | Positive Isolation |
| No. of Cells | Processes ∼2 x 109 cells total |
| Output Viability | >95% |
| Product Line | DYNAL™, Dynabeads™, FlowComp™ |
| Purity or Quality Grade | Research Grade |
| Quantity | 3 mL |
| Sample Type | PBMC, Blood |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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