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Invitrogen™ Dynabeads™ Untouched™ Human CD8 T Cells Kit
Description
Includes
10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD4, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)
The Dynabeads™ Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads™. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.
Advantages of the Dynabeads™ Untouched™ Human CD8 T Cells Kit:
• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required
Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Cell Type | T Cells (CD8) |
| Clonality | Monoclonal |
| Content And Storage | The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD4, CD14, CD16a, CD16b, CD19, CD36, CD56, CD123 and CD235a (Glycophorin A). Storage Conditions: 2°C to 8°C. Do not freeze. |
| Product Type | Cell Isolation Kit |
| Final Product Type | Cells |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Isolation Technology | Negative Isolation |
| No. of Cells | Processes ∼1 x 109 cells total |
| No. of Tests | 100 Isolations |
| Output Viability | >95%, >98% |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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