Invitrogen™ Dynabeads™ Untouched™ Human NK Cells Kit

Description

Includes

10mL Depletion MyOne; SA Dynabeads and 2mL Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A)

Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes.

Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched NK cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: NK Cells
• Content And Storage: The Kit Contains: 10 mL Depletion MyOne™ SA Dynabeads™ and 2 mL Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A).
  
  Store at 2°C to 8°C.
• Product Type: Cell Isolation Kit
• Final Product Type: Cells
• Format: Bead
• Isolation Technology: Negative Isolation
• No. of Cells: Processes ∼1 x 10⁹ cells/kit
• No. of Tests: 20
• Output Viability: >99%
• Product Line: DYNAL™, Dynabeads™, Untouched™
• Purity or Quality Grade: Research Grade
• Quantity: 1 kit
• Sample Type: MNC
• Shipping Condition: Room Temperature
• Starting Material Cell No.: ∼5 x 10⁷ cells/test
• Target Species: Human

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.