Invitrogen Dynabeads Untouched Human T Cells Kit
Use kit to isolate pure and viable untouched T-cells from PBMC by negative isolation
Manufacturer: Invitrogen 11344D
The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in sample and have not been in contact with Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
- High purity, recovery and viability of human untouched T cells
- Easy to use and to scale up
- No columns required
- Capacity: Processes approx. 1x109 cells total
- Cell Type: T Cells (Whole Population)
- Final Product: Cells
- Isolation Method: Negative Isolation
- Number of Rections: 100 Isolations
- Output Viability: >95%
- Sample Type: PBMC
- Starting Material (Cell No.): 1x107 PBMCs per isolation
- Target Species: Human
An antibody mix towards the non-T cells is added to sample and allowed to bind to cells. Dynabeads are added and will bind to antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in flow cytometer and used in any application.
A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended.
Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
Shipping Condition: Room Temperature
|Processes ~1x10^9 cells total|
|The Kit Contains: 10 ml Depletion Dynabeads® and 2 ml Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
Store at 2-8°C
|1x10^7 PBMCs per isolation|
|T Cells (Whole Population)|
|DYNAL®, Dynabeads®, Untouched™|
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only. Not for use in diagnostic procedures.