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Invitrogen™ DYNAL™ Dynabeads™ Untouched; Human T Cells Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Use kit to isolate pure and viable untouched T-cells from PBMC by negative isolation

Manufacturer:  Invitrogen™ 11344D

Catalog No. 11-344-D


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Includes: 10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)

Description

Description

The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in sample and have not been in contact with Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

  • High purity, recovery and viability of human untouched T cells
  • Easy to use and to scale up
  • No columns required
  • Capacity: Processes approx. 1x109 cells total
  • Cell Type: T Cells (Whole Population)
  • Final Product: Cells
  • Isolation Method: Negative Isolation
  • Number of Rections: 100 Isolations
  • Output Viability: >95%
  • Sample Type: PBMC
  • Starting Material (Cell No.): 1x107 PBMCs per isolation
  • Target Species: Human

Methodology:
An antibody mix towards the non-T cells is added to sample and allowed to bind to cells. Dynabeads are added and will bind to antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in flow cytometer and used in any application.

A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended.

Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation

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Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation
10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)
>95%
1 kit
Storage: 2°C to 8°C.
Human
1x107 PBMCs per isolation
Negative Isolation
PBMC
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For Research Use Only. Not for use in diagnostic procedures.