Invitrogen™ Dynabeads™ Untouched™ Human T Cells Kit  

Use kit to isolate pure and viable untouched T-cells from PBMC by negative isolation

Manufacturer:  Invitrogen™ 11344D

Catalog No. 11-344-D

Includes: 10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)

Description

The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in sample and have not been in contact with Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required
• Capacity: Processes approx. 1x10⁹ cells total
• Cell Type: T Cells (Whole Population)
• Final Product: Cells
• Isolation Method: Negative Isolation
• Number of Rections: 100 Isolations
• Output Viability: >95%
• Sample Type: PBMC
• Starting Material (Cell No.): 1x10⁷ PBMCs per isolation
• Target Species: Human

Methodology:
An antibody mix towards the non-T cells is added to sample and allowed to bind to cells. Dynabeads are added and will bind to antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in flow cytometer and used in any application.

A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended.

Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation

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Order Info

Shipping Condition: Room Temperature

Specifications

• Capacity: Processes ~1x10^9 cells total
• Content And Storage: The Kit Contains: 10 ml Depletion Dynabeads® and 2 ml Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
  Store at 2-8°C
• Isolation Technology: Negative Isolation
• Output Viability: >95%
• Shipping Condition: Room Temperature
• Starting Material Cell No.: 1x10^7 PBMCs per isolation
• Sample Type: PBMC

• Cell Type: T Cells (Whole Population)
• Final Product Type: Cells
• No. of Reactions: 100 Isolations
• Product Line: DYNAL®, Dynabeads®, Untouched™
• Species: Human
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.