Invitrogen™ Flp-In™-CHO Cell Line

Catalog No.	Quantity
R75807	1 mL

Catalog No. R75807 Supplier Invitrogen™ Supplier No. R75807

Description

Includes

3 x 10⁶ cells are supplied frozen in 1mL 90% complete medium and 10% DMSO.

Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector

• These cells contain single stably integrated FRT site at transcriptionally active genomic locus
• Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
• Created by transfecting parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones

• The Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones
• Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™)

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Content And Storage: 3 x 10⁶ cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
• Cell Line: Flp-In™-CHO Cell Line
• Species: Hamster
• Protein Tag: pSec
• Quantity: 1 mL
• Product Line: Flp-In

Frequently Asked Questions (FAQs)

Is multiple integration of the Flp-In expression construct possible? How do you screen for multiple integrants, and how stable is the Flp-In expression cell line?

In theory, one can get multiple integrations of the Flp-In expression construct—an FRT-specific integration event and a random, second-site integration. However, random integration is a relatively uncommon event. Limiting the amount of DNA in the transfection will reduce the chance of second-site integration. We have transfected 293 cells (lacking the FRT site) with the pcDNA5/FRT vector and have identified one potential second-site integrant after screening over 200 clones. DNA integrations can be detected by Southern blot. A single integrant will display a single band; double: two; triple: three, etc. We have maintained a number of Flp-In expression cell lines for over four months and have not observed any loss of the Flp-In expression construct, whether hygromycin selection was maintained or not.

What kind of Flp-In T-REx cell lines do you offer?

We offer the Flp-In T-REx system that contains pFRT/lacZeo and pcDNA6/TR stably integrated into HEK 293 cells. This cell line has been functionally tested for its ability to regulate expression.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.