Invitrogen™ Flp-In™-CHO Cell Line

Description

Includes

3 x 10⁶ cells are supplied frozen in 1mL 90% complete medium and 10% DMSO.

Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector

• These cells contain single stably integrated FRT site at transcriptionally active genomic locus
• Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
• Created by transfecting parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones

• The Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones
• Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™)

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Content And Storage: 3 x 10⁶ cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
• Cell Line: Flp-In™-CHO Cell Line
• Species: Hamster
• Protein Tag: pSec
• Quantity: 1 mL
• Product Line: Flp-In™

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.