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Invitrogen™ Flp-In™ Complete System
Description
Includes
The Flp-In Complete System includes the Core Kit (Cat. No. K601002), 1g Zeocin antibiotic, and 1g hygromycin B. All vectors are supplied supercoiled and lyophilized.
- Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
- Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site
- Major components include Flp-In. target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site
- Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter
- Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter
- Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In. host cell line, expresses CAT
Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | Flp-In Complete System |
| Selection Agent (Eukaryotic) | Zeocin™, Hygromycin |
| Content And Storage | The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag | Untagged |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 1 kit |
| Vector | pFRT |
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For Research Use Only. Not for use in diagnostic procedures.
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