Invitrogen™ Flp-In™ Core System

Description

Includes

The Flp-In Core Kit includes 20μg each of pFRT/lacZeo, pOG44, pcDNA5/FRT, and pcDNA5/FRT/CAT (expression control), and 2μg each of forward and reverse sequencing primers.

Contains necessary vectors that allow integration and expression of gene of interest in mammalian cells at specific genomic location

• For introduction of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
• Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Flp-In Core System
• Selection Agent (Eukaryotic): Zeocin™
• Content And Storage: The Flp-In™ Core Kit includes 20 μg each of pFRT⁄lacZeo, pOG44, pcDNA™5⁄FRT, and pcDNA™5⁄FRT⁄CAT (expression control), and 2 μg each of forward and reverse sequencing primers. Store at -20C.
• Protein Tag: Untagged
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pFRT
• Product Line: Flp-In™, pcDNA™
• For Use With (Application): Targeted Integration

For Research Use Only. Not for use in diagnostic procedures.