Invitrogen™ Flp-In™-Jurkat Cell Line

Description

Includes

3 x 10⁶ cells are supplied frozen in 1mL 90% complete medium and 10% DMSO.

Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector

• These cells contain single stably integrated FRT site at transcriptionally active genomic locus
• Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
• Created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones
• Co-transfection of Flp-InCell Lines with Flp-Inexpression vector and Flp recombinase vector, pOG44, results in targeted integration of expression vector to same locus in every cell, ensuring homogeneous levels of gene expression
• Flp-In-Jurkat cell lines work well with vectors that express gene from CMV promoter

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Content And Storage: 3 x 10⁶ cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
• Cell Line: Flp-In™-Jurkat Cell Line
• Species: Human
• Protein Tag: pSec
• Quantity: 1 mL
• Product Line: Flp-In™

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.