Invitrogen™ pcDNA™5/FRT/TO Vector Kit

Description

Includes

20μg of each: pcDNAFRT/TO and pcDNAFRT/TO/CAT vectors.

5.1kb inducible expression vector features multiple cloning site with 10 unique restriction sites to facilitate cloning gene of interest

• Control plasmid, pcDNA5/FRT/TO/CAT, is also included for use as positive control for transfection and expression in cell line of choice
• Hybrid human cytomegalovirus (CMV)/TetO2 promoter for high-level, tetracycline-regulated expression of gene of interest in wide range of mammalian cells
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of vector into Flp-InT-REx host cell line
• Hygromycin resistance gene for selection of stable cell lines

Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression, Targeted Integration

Specifications

• Constitutive or Inducible System: Inducible
• Delivery Type: Transfection
• Inducing Agent: Tetracycline
• Promoter: CMV/TO
• Product Type: Flp-In/T-REx System Expression Vector
• Selection Agent (Eukaryotic): Hygromycin
• Content And Storage: Contains 20 μg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors. Store both vectors at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Protein Tag: Untagged
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pcDNA
• Product Line: Flp-In™, T-REx™, pcDNA™
• For Use With (Application): Regulated Expression

For Research Use Only. Not for use in diagnostic procedures.