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Invitrogen™ FreeStyle™ 293-F Cells

Catalog No. R79007
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Quantity:
1 mL

Description

Complete cell culture system for generating large amounts of mammalian recombinant protein

Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle MAX 293 Expression System or FreeStyle 293 Expression System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle 293 Expression Medium. The FreeStyle 293-F cell line is supplied in a vial containing 1mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle 293 Expression Medium and 10% DMSO

Characteristics of FreeStyle 293-F cells:

  • Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were recloned by limiting dilution
  • The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages
  • Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle 293 Expression Medium
  • Demonstrate high transfection efficiencies with FreeStyle MAX reagent
  • Suspension cultures may be transfected in FreeStyle 293 Expression Medium without the need to change media
  • Permits transfection of cells at large volumes
  • Voltage limit: 0.5-2.5kV

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection

TRUSTED_SUSTAINABILITY

Specifications

Cell Line FreeStyle™ 293-F Cells
Content And Storage 1 vial of 1 x 107 cells. Store in liquid nitrogen.
Cell Type 293-F
Species Human
For Use With (Application) Protein Expression and Transfection
Media Adaptation FreeStyle™ 293 Expression Medium
Product Line Freestyle™
Product Type 293-F Cell
Quantity 1 mL
System Type FreeStyle™

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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