Invitrogen™ FreeStyle™ MAX CHO Expression System

Description

Rapid and high-yield mammalian protein production

FreeStyle MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle cells with high efficiency. The FreeStyle MAX Expression System combines GIBCO™ FreeStyle Media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO₂ incubator and a platform shaker with shake flasks or a spinner culture.

• Easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
• Simplified downstream purification of secreted proteins with FreeStyle serum-free medium
• Straightforward protocol for production scale-up
• Replace time-consuming stable cell line generation with large-scale functional protein production in one week

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection, Transient Protein Production and High-Throughput Screening

Specifications

• Cell Line: FreeStyle™ CHO-S Cells
• Classification: Animal Origin-Free, Serum-Free
• Content And Storage: Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.
• Delivery Type: Transfection
• Culture Type: Suspension Cell Culture
• Cell Type: CHO
• Transfection Technique: Lipid-based Transfection
• Expression Mechanism: Cell-Based Expression
• Expression System: Mammalian
• For Use With (Application): Protein Expression
• Key Functions: Protein Production
• Product Line: Freestyle™
• Product Type: Expression System
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.