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Invitrogen™ β-Gal Staining Kit

Catalog No. K146501
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Catalog No. K146501 Supplier Invitrogen™ Supplier No. K146501
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Description

Includes

Sufficient reagents to stain fifty 60mM plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10μg of pcDNA 3.1/His/lacZ control vector.

Determine percentage of transfected cells expressing lacZ

  • lacZ is bacterial gene often used as reporter construct in eukaryotic transfection experiments
  • Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
  • β-galactosidase catalyzes hydrolysis of X-gal, producing a blue color that can be easily visualized under microscope
  • Colorimetric detection

Beta-Galactosidase Assays/Enzyme and Protein Activity Assays/Proteins, Expression, Isolation and Analysis/Reporter Assays

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 μg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Assay β-Gal (lacZ) Assay
Detection Method Colorimetric
Product Type Beta-Gal Staining Kit
Substrate X-Gal
Quantity 1 Kit
Target Beta-Galactosidase

Frequently Asked Questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

For Research Use Only. Not for use in diagnostic procedures.

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