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Invitrogen™ Baculovirus Expression System with Gateway™ Technology
Description
Includes
LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.
- Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
- pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification
Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | Polyhedrin |
| Product Type | Expression System |
| Content And Storage | The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored. |
| Protein Tag | GST Tag, His Tag (6x), Untagged |
| Cloning Method | Gateway |
| Quantity | 20 reactions |
| Vector | pDEST, Gateway Baculovirus Vectors |
| Product Line | Gateway™ |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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