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Invitrogen™ Baculovirus Expression System with Gateway™ Technology

Catalog No. 11827011
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20 reactions
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11-827-011 20 reactions
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Catalog No. 11-827-011 Supplier Invitrogen™ Supplier No. 11827011
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Description

Includes

LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.

Designed to create recombinant pFastBac™ plasmids containing polyhedrin promoter

  • Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
  • pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification

Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Promoter Polyhedrin
Product Type Expression System
Content And Storage The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.
Protein Tag GST Tag, His Tag (6x), Untagged
Cloning Method Gateway
Quantity 20 reactions
Vector pDEST, Gateway Baculovirus Vectors
Product Line Gateway

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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