Invitrogen Gateway Baculovirus Expression System
Designed to create recombinant pFastBac plasmids containing polyhedrin promoter
Manufacturer: Invitrogen 11827011
- Destination vectors carrying polyhedrin promoter for production of native (pDEST8), N-terminal histidine fusion (pDEST10), and N-terminal GST fusion (pDEST20) proteins
- pDEST10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification
Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis
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|The Baculovirus Expression System with Gateway Technology includes LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.|
|Store LR Clonase enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.|
|Gateway Baculovirus Vectors, pDEST|
|GST Tag, His Tag (6X), Untagged|
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For Research Use Only. Not for use in diagnostic procedures.