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Invitrogen™ Gateway™ Baculovirus Expression System Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Designed to create recombinant pFastBac™ plasmids containing polyhedrin promoter

Manufacturer:  Invitrogen™ 11827011

Catalog No. 11-827-011


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Includes: LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2µg/µL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.

Description

Description

  • Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
  • pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification

Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis

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Specifications

Specifications

The Baculovirus Expression System with Gateway Technology includes LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.
20 reactions
Store LR Clonase enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.
Polyhedrin
Gateway Baculovirus Vectors, pDEST
GST Tag, His Tag (6X), Untagged
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For Research Use Only. Not for use in diagnostic procedures.