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Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Description
Includes
Gateway BP Clonase II enzyme mix and reaction buffer, Gateway pDONR;221 or pDONR;/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and OmniMAX2 Competent Cells.
- Designed to create entry clone following PCR with attB-modified custom primers
- With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
- PCR product is directionally cloned with efficiencies typically >99%
- Rapid, efficient, directional cloning of PCR products
- Versatility to use any amplification enzyme
- Cloning without need for restriction endonucleases or ligase
- Selection of entry clones with kanamycin or Zeocin™
- In addition, the pDONR221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use
Cloning,Gateway Cloning

Specifications
Specifications
Cloning Method | Gateway™ |
Content And Storage | The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reaction buffer, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C. |
Product Line | Gateway™, OmniMAX™, pDONR™ |
Product Type | PCR Cloning System |
Quantity | 20 Reactions |
Vector | pDONR |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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