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Invitrogen™ pAd/PL-DEST™ Gateway™ Vector Kit

Catalog No. V49420
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6 μg
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V49420 6 μg
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Catalog No. V49420 Supplier Invitrogen™ Supplier No. V49420
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Description

Includes

pAd/PL-DEST Vector 150ng/μL in TE Buffer, pH 8.0; 40μL pAd/CMV/V5-GW/lacZ control plasmid 1μg/μL in TE Buffer, pH 8.0, 10μL.

For easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and nondividing mammalian cells

  • The vector allows generation of an adenovirus containing the target gene
  • Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters
  • High efficiency and rapid recombination cloning
  • Produces high titer adenoviral stocks
  • Efficient delivery of the gene to dividing and nondividing mammalian cells in vitro or in vivo
  • Produces replication-incompetent virus for enhanced biosafety of the system
  • Amenable for use in high-throughput applications
  • Promoterless vector allows gene of interest to be controlled by a promoter of choice
  • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
  • Ampicillin selection marker

Antibody and Cell Culture Production, Bioproduction, Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery, Viral Vaccine Production

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Adenoviral
Promoter None (Promoterless)
Product Type Adenovirus Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage • pAd⁄PL-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)
Protein Tag Untagged
Cloning Method Gateway
Quantity 6 μg
Vector pDEST
Product Line Gateway, ViraPower
For Use With (Application) Clone your own Promoter, Viral Expression
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For Research Use Only. Not for use in diagnostic procedures.

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