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Invitrogen™ Gateway™ pcDNA™-DEST47 Vector 
Designed for high-level, constitutive expression in variety of mammalian cell lines
Manufacturer: Invitrogen™ 12281010
Includes: All destination vectors are provided lyophilized and supercoiled.
Requires : Gateway entry clone, appropriate Gateway LR Clonase™ enzyme mix, and reaction buffer.
Description
- C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein
- Cytomegalovirus (CMV) promoter for high-level expression
- attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
- Neomycin resistance gene for stable selection
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. Coli
Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression, Proteins, Expression, Isolation and Analysis
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Specifications
| Constitutive | |
| CMV | |
| GFP (Cycle 3) | |
| Constitutive Expression | |
| GFP (Cycle 3) | |
| pDEST, pcDNA |
| Transfection | |
| Gateway System Destination Expression Vector | |
| Geneticin™ (G-418) | |
| Gateway™, pcDNA™ | |
| Gateway™ |
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.