Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R Chemically Competent E. coli (Supply Center Only)

Description

Includes

TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli or Mach1-T1^R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Designed for cloning PCR products directly from PCR reaction in just 5 minutes

• Use pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants
• pCR™8/GW/TOPO has:3'-T overhangs for direct ligation of Taq-amplified PCR products
• att sites for rapid recombination into variety of Gateway destination vectors
• Pimer sites, within att regions, located less than 55 base pairs from PCR product insertion site for convenient sequencing
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Order Info

Shipping Condition: Dry Ice

Specifications

• Cloning Method: TOPO™ with Gateway™
• Content And Storage: The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli or One Shot™ Mach1™-T1^R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• No. of Reactions: 20 reactions
• Product Line: One Shot™
• Product Type: TA Cloning Kit
• Quantity: 20 Reactions
• Shipping Condition: Dry Ice
• Vector: pCR

For Research Use Only. Not for use in diagnostic procedures.