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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R Chemically Competent E. coli (Supply Center Only)

Catalog No. K252020SC
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Catalog No. K252020SC Supplier Invitrogen™ Supplier No. K252020SC
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Description

Includes

TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli or Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Designed for cloning PCR products directly from PCR reaction in just 5 minutes

  • Use pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants
  • pCR™8/GW/TOPO has:3'-T overhangs for direct ligation of Taq-amplified PCR products
  • att sites for rapid recombination into variety of Gateway destination vectors
  • Pimer sites, within att regions, located less than 55 base pairs from PCR product insertion site for convenient sequencing
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Cloning Method TOPO™ with Gateway™
Content And Storage The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli or One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
No. of Reactions 20 reactions
Product Line One Shot
Product Type TA Cloning Kit
Quantity 20 Reactions
Shipping Condition Dry Ice
Vector pCR

For Research Use Only. Not for use in diagnostic procedures.

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