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Invitrogen™ Gateway™ pDONR™221 Vector

Catalog No. 12536017
Encompass_Preferred
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Quantity:
6 μg

Includes

6μg pDONR221 vector, lyophilized in TE buffer, pH 8.0.

Enables rapid cloning of one or more genes into virtually any protein expression system

  • Designed to create entry clone following PCR with attB-modified custom primers
  • With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
  • PCR product is directionally cloned with efficiencies typically >99%
  • Rapid, efficient, directional cloning of PCR products
  • Versatility to use any amplification enzyme
  • Cloning without need for restriction endonucleases or ligase
  • Selection of entry clones with kanamycin or Zeocin™
  • In addition, pDONR221 and pDONR™/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use

Cloning, Gateway Cloning

TRUSTED_SUSTAINABILITY

Specifications

Product Type Gateway System Destination Expression Vector
Content And Storage 6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.
Cloning Method Gateway
Quantity 6 μg
Vector pDONR
Product Line Gateway™, pDONR™
WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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