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Invitrogen™ Gateway™ pDONR™221 Vector
Enables rapid cloning of one or more genes into virtually any protein expression system
Supplier: Invitrogen™ 12536017
Includes: 6μg pDONR221 vector, lyophilized in TE buffer, pH 8.0.
Description
- Designed to create entry clone following PCR with attB-modified custom primers
- With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
- PCR product is directionally cloned with efficiencies typically >99%
- Rapid, efficient, directional cloning of PCR products
- Versatility to use any amplification enzyme
- Cloning without need for restriction endonucleases or ligase
- Selection of entry clones with kanamycin or Zeocin™
- In addition, pDONR221 and pDONR™/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use
Cloning, Gateway Cloning
Specifications
| Gateway System Destination Expression Vector | |
| Gateway | |
| Gateway™, pDONR™ |
| 6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C. | |
| pDONR |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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