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Invitrogen™ Gateway™ pET-DEST42 Vector
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Quantity:
6 μg
Description
Includes
The Champion pET-DEST42 Gateway destination vector includes 6μg of vector.
- Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
- Bacteriophage T7lac promoter for high-level expression
- O operator sequences for lac repressor binding to ensure tighter regulation of transcription
- pBR322 ori for minimal basal expression
- C-terminal V5 and 6xHis tags for efficient detection and purification
- R sites for efficient recombination with any attL-flanked Gateway entry vector
Bacterial Expression, Bioproduction, Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Constitutive or Inducible System | Inducible |
| Inducing Agent | IPTG |
| Promoter | T7, lacO |
| Product Type | Gateway System Destination Expression Vector |
| Selection Agent (Eukaryotic) | None |
| Content And Storage | The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Gateway |
| Quantity | 6 μg |
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Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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