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Invitrogen™ Gateway™ pET-DEST42 Vector

Catalog No. 12276010
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12-276-010 6 μg
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Catalog No. 12-276-010 Supplier Invitrogen™ Supplier No. 12276010
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Description

Includes

The Champion pET-DEST42 Gateway destination vector includes 6μg of vector.

Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by strong bacteriophage T7 promoter

  • Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
  • Bacteriophage T7lac promoter for high-level expression
  • O operator sequences for lac repressor binding to ensure tighter regulation of transcription
  • pBR322 ori for minimal basal expression
  • C-terminal V5 and 6xHis tags for efficient detection and purification
  • R sites for efficient recombination with any attL-flanked Gateway entry vector

Bacterial Expression, Bioproduction, Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent IPTG
Promoter T7, lacO
Product Type Gateway System Destination Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Gateway
Quantity 6 μg
Vector pET, pDEST
Product Line Gateway
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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