Invitrogen™ pLenti6.2-GW/EmGFP Expression Control Vector

Description

Includes

pLenti6.2-GW/EmGFP Vector: 20μg of control vector is supplied in solution as 40μL of 0.5μg/μL in 10mM Tris–HCl, 1mM EDTA, pH 8.0.

Designed as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells

• Also a titer control to produce an EmGFP-expressing lentivirus stock and a transduction control following transduction in both dividing and nondividing mammalian cells
• This is not a cloning vector
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP
• PKG Promoter for expression of Blasticidin selection marker

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Cloning, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP), Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Product Type: Lentiviral Expression Vector
• Reporter Gene: GFP (EmGFP)
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: pLenti6.2-GW⁄EmGFP Vector: 20 μg of control vector is supplied in solution as 40 μl of 0.5 μg⁄μl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.
• Selection Marker Promoter: PGK Promoter
• Protein Tag: Untagged
• Cloning Method: Gateway
• Quantity: 1 Ea.
• Vector: pLenti
• Product Line: Gateway™, ViraPower™, Vivid Colors™
• For Use With (Application): Reporter Assays, Viral Expression

For Research Use Only. Not for use in diagnostic procedures.