Invitrogen™ pLenti6/UbC/V5-DEST™ Gateway™ Vector

Description

Includes

pLenti6/UbC/V5-DEST 6μg, pLenti6/UbC/V5-GW/lacZ Control vector: 10μg; Stbl3 Chemically Competent E. coli(Box 2), Stbl3 Cells: 21 x 50μL, pUC19 control DNA (10pg/μL): 50μL, S.O.C. Medium: 6mL.

For lentiviral-based expression of a target gene in dividing and nondividing mammalian cells

• The vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection
• Suited to long-term experiments
• Accurate titer of functional virus
• V5 epitope tag at C terminus

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: UbC
• Product Type: Lentiviral Expression Vector
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: Vector Kit (Box 1)
  pLenti6⁄UbC⁄V5-DEST™:6 μg, store at -20°C
  pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector: 10 ug, store at -20°C
  
  One Shot™ Stbl3™ Chemically Competent E. coli(Box 2)
  Stbl3™ Cells: 21 x 50 μL, store at -80°C
  pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C
  S.O.C. Medium: 6 mL, store at -80°C
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 6 μg
• Vector: pDEST, pLenti
• Product Line: Gateway™, ViraPower™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.