Invitrogen™ pTrcHis2 A, B, & C Bacterial Expression Vectors

Description

Includes

All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided.

Offer enhanced translation initiation and high-level expression in E. coli

• These vectors feature high-level regulated transcription from the trc promoter
• Enhanced translation efficiency of eukaryotic genes in E. coli
• The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
• This particular vector offers C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
• C-terminal Xpress epitope for easy detection with an anti-Xpress antibody
• Enterokinase cleavage site for removal of fusion tag

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Inducing Agent: IPTG
• Promoter: Trc, lacO
• Product Type: Bacterial Expression Vector
• Selection Agent (Eukaryotic): None
• Content And Storage: All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cleavage: EK (Enterokinase) Recognition Site
• Protein Tag: Thioredoxin
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pTrc

For Research Use Only. Not for use in diagnostic procedures.