Invitrogen™ T-REx™ Core Kit, with pcDNA™4/TO Vector

Catalog No.	Quantity
K102002	1 kit

Catalog No. K102002 Supplier Invitrogen™ Supplier No. K102002

Description

Includes

20μg of pcDNA4/TO or 20μg each of pcDNA;4/TO/myc-His A, B, and C; a positive control vector, pcDNA;6/TR, and forward and reverse sequencing primers. All vectors are supercoiled and provided lyophilized.

Regulated mammalian expression system yields exceptionally high levels of induced expression

 

• Uses complete CMV promoter and adds control elements from bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of strongest mammalian promoter sequences known
• Specific Activation: uses a repressor mechanism that blocks transcription from powerful CMV promoter in absence of tetracycline
• Because elements do not use viral transactivators, you can achieve high-level expression from complete CMV promoter without secondary, non-specific activation of host genes
• T-REx transcriptional control elements are provided through two tetracycline operator sequences (TetO₂) that have been inserted between the TATA box of the CMV promoter and transcriptional start site
• TetO₂ sequence itself has no effect on expression
• Tetracycline repressor protein (TR) is present, it effectively binds TetO₂ sites and blocks transcription initiation
• Tetracycline added to culture medium binds to, and changes conformation of, TR protein; Change causes TR protein to release TetO₂ sites, derepressing transcription from CMV promoter; The result is high-level expression of the gene of interest

 

 

• Expression levels can be modulated based on tetracycline concentration and can be induced to levels that are achieved with constitutive CMV expression vectors
• T-REx is powerful inducible mammalian expression system that allows to regulate expression from complete human cytomegalovirus (CMV) enhancer-promoter
• Complete CMV enhancer-promoter sequence containing two copies of tetracycline operator TetO₂ sequence for high-level regulated expression
• Zeocinor hygromycin resistance gene for effective selection of stable mammalian cell lines
• Large multiple cloning site to simplify cloning
• In addition, pcDNA™4/TO/myc-His offers a c-myc epitope for rapid detection of recombinant protein with an Anti-myc Antibody and a polyhistidine (6xHis) sequence for simple purification of recombinant protein with nickel-chelating resin and detection with Anti- His(C-term) Antibody
• Regulatory vector, pcDNA™6/TR, is provided for high-level expression of tetracycline repressor (TR) protein
• Vector expresses Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express TR protein

 

Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Regulated Expression

Specifications

• Constitutive or Inducible System: Inducible
• Delivery Type: Transfection
• Inducing Agent: Tetracycline
• Promoter: CMV/TO
• Product Type: T-Rex Core Cloning Kit with Vector
• Selection Agent (Eukaryotic): Zeocin™, Blasticidin
• Content And Storage: The T-REx™ Core Kit includes 20 μg of pcDNA™4/TO or 20 μg each of pcDNA™4/TO/myc-His A, B, and C; a positive control vector, pcDNA™6/TR, and forward and reverse sequencing primers.
  
  All vectors are supercoiled and provided lyophilized.
  
  Store all components at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: Untagged
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pcDNA
• Product Line: Gateway, T-REx, pcDNA
• For Use With (Application): Regulated Expression

For Research Use Only. Not for use in diagnostic procedures.