Invitrogen™ ViraPower™ II Lentiviral Gateway™ Expression System

Description

Includes

6μg of pLenti6.2/V5-DEST vector (40μL vector at 150ng/μL in TE Buffer, pH 8.0), 10μg of pLenti6.2/V5-GW/lacZ plasmid (20μL of plasmid at 0.5μg/μL in TE Buffer, pH 8.0), Stbl3 Chemically Competent E. coli; 21 x 50μL of Stbl3 Cells, 6mL of S.O.C.Medium and 50μL of pUC19 control DNA (10pg/μL); ViraPower Bsd Lentiviral Support Kit includes 195μg of ViraPower Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1μg/μL in TE Buffer, pH 8.0), 0.75mL of Lipofectamine 2000, 50mg of Blasticidin powder; 293FT Cell Line, 1 vial containing 3 X 10⁶frozen cells in 1mL of Freezing Medium

Includes all components needed to generate lentivirus

• Generates replication-incompetent lentivirus for transducing dividing and nondividing mammalian cells
• The pLenti6.2/V5-DESTvector in kit has CMV promoter for driving constitutive expression of target gene in mammalian cells
• Uses the PGK promoter instead of SV40 promoter in lentiviral vector for driving long-term, persistent expression of blasticidin stable selection marker
• Easy recombination-based cloning and stable expression, long term expression
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Product Type: Lentiviral Expression System
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit
  • 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)
  • 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
  • One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).
  
  ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
  • 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)
  • 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
  • 50 mg of Blasticidin powder (Cat # R21001)
  • 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 10⁶frozen cells in 1 mL of Freezing Medium.
  
  Storage
  • Vectors: -20°C
  • ViraPower™ Packaging Mix: -20°C
  • Blasticidin: -20°C
  • Lipofectamine™ 2000: 4°C (do not freeze)
  • One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
  • 293 FT Cells: Liquid Nitrogen
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 1 kit
• Vector: pLenti
• Product Line: Gateway™, ViraPower™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.