Invitrogen™ ViraPower™ UbC Lentiviral Gateway™ Expression Kit

Description

Includes

pLenti6/UbC/V5-DEST (Box 1): 6μg; pLenti6/UbC/V5-GW/lacZ Control vector (Box 1): 10μg; Stbl3 Chemically Competent E. coli (Box 2): 21 x 50μL; pUC19 control DNA (10pg/μL): 50μL; S.O.C. Medium: 6mL; Support kit (Box 3); ViraPower Packaging Mix (1μg/μL): 195μg; Lipofectamine 2000: 0.75mL; Blasticidin: 50mg; 293 FT cells (3 x 10⁶/mL) (Box 4): 1mL

Facilitates easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and nondividing mammalian cells

• Contains all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit
• The pLenti6/UbC/V5-DEST vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells
• Well-suited to long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway recombination cloning technology
• Vector includes UbC promoter and V5 epitope tag at C terminus

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: UbC
• Product Type: Lentiviral Expression Kit
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: Vector kit (Boxes 1 and 2)
  pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
  pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
  One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
  pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
  S.O.C. Medium: 6 mL -80°C
  
  Support kit (Box 3)
  ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
  Lipofectamine™ 2000: 0.75 mL, +4°C
  Blasticidin: 50 mg, -20°C
  293 FT cells (3 x 10⁶⁄mL) (Box 4): 1 mL, liquid nitrogen
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 1 kit
• Vector: pLenti
• Product Line: Gateway™, ViraPower™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.