Learn More
Invitrogen™ InsectSelect™ Glow Kit with Sf9 Cells
Description
Includes
The InsectSelect Glow Kit includes 20μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1g Zeocin, Cellfectin Reagent, Sf9 cells, and 1L of GIBCO Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described.
- The pIZT/V5-His vector features the OpIE2 promoter for constitutive expression
- The Zeocin resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin
- pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistanc protein (Zeo-GFP): The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells
Constitutive Protein Expression in Insect Cells, High-level Protein Production in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | OplE2 |
| Product Type | Sf9 Cells Glow Kit |
| Content And Storage | The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag Position (to your gene) | C-terminal |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 1 kit |
| Vector | pIZ |
| Product Line | InsectSelect™ |
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.