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Invitrogen™ IPTG

Catalog No. 15529019
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Quantity:
1 g

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.

IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures. IPTG binds to the lac repressor, releasing it from the lac operator, and thereby allowing the transcription of genes in the lac operon, such as the gene coding for β-galactosidase.

In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction in E. coli. Though mainly used in cell culture assays, publications demonstrated the use IPTG in vivo in animal models too.

Primary applications
• Blue-white screening—induce functional β-galactosidase
• Induce protein expression from inducible T7 RNA polymerase E. coli expression systems egulated by lac operon

Find the media format that fits your needs
• We offer a wide variety of ready-to-use media formulations, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) or S.O.C recovery medium (Cat. No. 15544034).
• X-Gal (Cat. No. 15520018) or Bluo-Gal (Cat. No. 15519028) for blue-white screening.

Order Info

Approved for shipment at Room Temperature or on Wet Ice.

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material IPTG (Isopropyl β-D-thiogalactopyranoside)
Content And Storage • IPTG Powder (1 g)

Store at –20°C.

Format Bottle
Quantity 1 g
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Form Powder
WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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