Invitrogen™ MagicMedia™ E. coli Expression Medium

Catalog No.	Quantity
K6803	1 L

Catalog No. K6803 Supplier Invitrogen™ Supplier No. K6803

Description

Includes

Contain proprietary component A (in SoluPouchpowder or 1X liquid format) and component B (in buffer format).

Specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coliexpression systems without monitoring culture O.D. (optical densities) or adding inducer

• The proprietary formulation enables E. coligrowth to reach culture densities 3 to 10-fold higher than those achieved with traditional LB + IPTG methods
• Achieve 3 to 10 times higher protein yields with much less hands-on time
• Eliminate time-consuming O.D. monitoring steps: inoculate prepared MagicMediaE. coliExpression Medium, grow the culture overnight, and harvest
• Reduce culture volume to simplify handling and scale-up processes
• Excellent for simultaneous expression of multiple clones for high-throughput applications

Bioproduction, Cloning, Transformation

Order Info

Shipping Condition: Room Temperature

Specifications

• Format: Liquid
• Product Type: E. Coli Expression Medium
• Content And Storage: Component A (1X liquid) and Component B (liquid)
  
  Store in the dark at 2−8°C.
  
  Shelf life: 12 months from date of manufacture
• Shipping Condition: Room Temperature
• Target Organism Class: E. coli
• Quantity: 1 L
• Product Line: MagicMedia

Frequently Asked Questions (FAQs)

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

How does expression in MagicMedia affect the solubility of proteins when compared to standard LB and IPTG induction?

In most cases, the ratio of soluble to insoluble proteins is not much different when compared to LB and IPTG. However, since more total protein is typically obtained in MagicMedia, one also obtains more total soluble protein.

How does MagicMedia compare to LB-IPTG with regards to expression of toxic proteins?

Toxic protein expression has not been investigated using MagicMedia. However, the Dual Temperature protocol provided in the manual can be tried. This may be a situation where LB/IPTG induction has an advantage since parameters (time, temperature, and IPTG concentration) are easily adjusted for optimal expression.

Does MagicMedia have options to express and induce at mid-log phase?

No. Protein expression in MagicMedia occurs gradually over an 18-24 hour period, with detectable levels of expression beginning 6 hrs after a 1:40 seed inoculation with incubation at 30°C, 300 rpm.

Can temperatures lower than 30°C be used with MagicMedia even though the suggested incubation temperatures are 37°C and 30°C?

Yes, expression of proteins at 18°C and 25°C has been validated using the Dual Temperature protocol as follows. Day 1: Inoculate LB + antibiotic with an expression colony and incubate overnight at 30°C, 300 rpm. Day 2: Inoculate pre-warmed (30°C) MagicMedia + antibiotic at 1:40 with overnight seed culture. Incubate culture at 30°C, 300 rpm for 6-7 hours or until OD600 reaches >6. Transfer culture to either 18°C or 25°C, 300 rpm and incubate for 24-36 hours. Day 3: Harvest and analyze results.

For Research Use Only. Not for use in diagnostic procedures.