Invitrogen™ Mouse Cot-1 DNA

Catalog No.	Quantity
18-440-016	500 μg

Catalog No. 18-440-016 Supplier Invitrogen™ Supplier No. 18440016

Description

Includes

Mouse Cot-1 DNA™ is supplied at 1mg/mL in 10mM Tris-HCl (pH 7.4), 1mM EDTA.

Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members

Mouse Cot-1 DNA™ is commonly used to block non-specific hybridization in microarray screening. It can also be used to suppress hybridization of rodent repetitive DNA sequences when mapping rodent clones by in situ hybridization or Southern blotting and to identify mouse clones in screens of somatic-cell hybrid libraries derived from mouse-hamster hybrid cells (1-3). The amount supplied is sufficient for 5 to 10 Southerns or 500 in situ hybridizations.

Performance and Quality Testing: Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Mouse Cot-1 DNA™ 1:100 in 50mM NaOH and using the conversion factor 0.033μg/μL/A 260 . Other methods used to determine concentration may yield varying results.

Array CGH, Cell Analysis, Cellular Imaging, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Product Type: Mouse DNA
• Content And Storage: Mouse Cot-1 DNA™ is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA. Store at -20°C.
• Reagent Type: Cot-1 DNA
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Species: Mouse
• Product Line: Cot-1 DNA
• For Use With (Application): Block Non-Specific Hybridization
• Quantity: 500 μg
• Form: Liquid

Frequently Asked Questions (FAQs)

How much COT-1 DNA should I use to suppress repetitive sequences during hybridization?

For Southern blot hybridizations, add 50 µg of COT-1 DNA (at 10 µg/µL) to 50 µL of 20X SSC, 25 µL distilled water and 20 µL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe. For in situ hybridizations, combine genomic probe with the proper amount of COT-1 DNA such that the final concentration of COT-1 DNA is 0.3 µg/µL for cosmid, plasmid, and lambda probes; or, at 1 µg/µL for Alu PCR probes. Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 degrees C for 5 min. Incubate at 37 degrees C for 5 to 15 min.

How can I label COT-1 DNA?

Probes can be labeled with 32P by random primer or nick translation procedures using the Random Primers DNA Labeling System (Cat. No.18187-013) or Nick Translation System (Cat. No. 18160-010). Biotinylated COT-1 DNA can be prepared by nick translation with the BioNick Labeling System (Cat. No. 18247-015) or by the BioPrime DNA Labeling System (Cat. No. 18094-011). Improved results can be obtained when the COT-1 DNA is first ligated to itself to provide an optimum template.

For Research Use Only. Not for use in diagnostic procedures.