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Invitrogen™ MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector
Description
Includes
Include vectors and primers, Mach1 T1R Chemically Competent Cells, and LR Clonase II Plus and BP Clonase II enzymes.
- Using specifically designed att sites for recombinational cloning, clone two, three, or four DNA fragments into any Gateway Destination vector containing attR1 and attR2 sites
- Resulting expression clone is ready for downstream expression and analysis applications
- Clone multiple DNA fragments into one vector without using restriction enzymes or ligases
- Full advantage of wide selection of Gateway Destination vectors or create vectors
- Replace multiple plasmid transfections with single vector carrying all DNA elements of interest
- MultiSite Gateway Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway Destination vector
Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Multi-Gene Expression, Multisite Gateway, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | None (Promoterless) |
| Product Type | Gateway System Destination Expression Vector |
| Content And Storage | The MultiSite Gateway™ Pro Kits include vectors and primers as indicated in Table 1, One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes. Store cells at -80°C. Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Cloning Method | Gateway |
| Quantity | 20 reactions |
| Vector | MultiSite Gateway Vectors |
| Product Line | Gateway™ |
| For Use With (Application) | Clone your own Promoter, Multi-Gene Expression |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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