Invitrogen™ Neon™ Transfection System

Catalog No.	Quantity
MPK5000	1 Ea.

Catalog No. MPK5000 Supplier Invitrogen™ Supplier No. MPK5000

Description

Includes

Neon electroporation device, pipette, pipette station, USB thumbdrive, manual and power cord.

For stem cells, primary cells, hard-to-transfect cells and established cell lines

Enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 10⁴ cells per reaction and a reaction time of just 1 second. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability.

• Up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Easily transfect from 2 × 10⁴ cells to 6 × 10⁶ cells per reaction
• Easy to use, with a single reagent kit for all cell types
• Open system allows electroporation parameters to be optimized freely
• One-of-a-kind electroporation
• Plug-n-play out of the box
• Perform more transfections with limited or precious cells

For use at room temperature under normal laboratory conditions.

Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Noncoding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA

Order Info

Shipping Condition: Room Temperature

Specifications

• Amperage: 3.0-6.0 A
• Classification: Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
• Content And Storage: The Neon™ Transfection System comes with a Neon™ electroporation device, pipette, pipette station, USB thumbdrive, manual and power cord.
  
  For use at room temperature under normal laboratory conditions.
• Cell Type: Stem Cells, Primary Cells, Hard-to-Transfect Cells
• Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
• Sample Type: Plasmid DNA, Synthetic siRNA
• For Use With (Equipment): Neon™ Transfection System
• For Use With (Application): Transfection
• Max. Voltage: 0.5-2.5 kV
• Product Line: Neon
• Product Type: Transfection System
• Purity: Transfection Grade
• Quantity: 1 Ea.
• Reaction Time: 1 sec
• Serum Compatible: Yes
• Shipping Condition: Room Temperature
• Starting Material Amount: 2x10^4 - 6 x10^8 cells; Varies by cell type
• System Type: Neon™ Transfection System
• Voltage: 100-240 VAC
• Wattage: 300 W
• Volume (Metric) Sample: Depends on kit used

Frequently Asked Questions (FAQs)

Can Lipofectamine LTX be used for transfecting primary cells?

We have tested four primary cell lines: HuVec, NHFF (Normal human foreskin fibroblast), MJ90 and NDHF. We have observed transfection efficiency in the range of 5-25%. We suggest optimizing transfection efficiency for your primary cell line by varying DNA and lipid ratio. If you are using any other primary cell line, please follow guidelines suggested in the product insert. Alternatively, Neon electroporation system and viral delivery methods are recommended.

Can Lipofectamine LTX be used in transfection of Suspension cells and other hard to transfect cells?

We have tested transfection of suspension cells such as Jurkat and K562. We achieved transfection efficiency of less than 5% for each of them. We do not notice toxicity with Lipofectamine LTX reagent when used with these suspension cells. For other cell lines please follow the guidelines in the product insert. However, if you experience low transfection efficiency, then we recommend Neon electroporation system or Viral delivery.

For Research Use Only. Not for use in diagnostic procedures.