Learn More
Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit

Description
Includes
GFP Fusion TOPO Expression Kits contain two boxes. The GFP Fusion TOPO TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. The One Shot™ box contains transformation reagents including single-use, 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.
- Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vector
- Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
- Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
- Expression is easily detected in living cells using fluorescence
- Recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum
Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications
Specifications
Constitutive or Inducible System | Constitutive |
Delivery Type | Transfection |
Promoter | CMV |
Product Type | TOPO Expression Kit |
Reporter Gene | GFP (Cycle 3) |
Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
Content And Storage | GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored. |
Protein Tag | GFP (Cycle 3) |
Cloning Method | TOPO-TA |
Quantity | 20 reactions |
Show More |
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.