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Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit

Catalog No. K481001
Encompass_Preferred
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Quantity:
20 reactions

Includes

GFP Fusion TOPO Expression Kits contain two boxes. The GFP Fusion TOPO TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. The One Shot™ box contains transformation reagents including single-use, 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.

Designed to allow fusion of protein of interest to Cycle 3 GFP protein

  • Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vector
  • Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
  • Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
  • Expression is easily detected in living cells using fluorescence
  • Recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type TOPO Expression Kit
Reporter Gene GFP (Cycle 3)
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.
Protein Tag GFP (Cycle 3)
Cloning Method TOPO-TA
Quantity 20 reactions
Vector TOPO-TA Vectors
Product Line TOPO™
For Use With (Application) Reporter Assays
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For Research Use Only. Not for use in diagnostic procedures.

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