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Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit

Description
Includes
All of the reagents necessary for PCR (except Taq polymerase) including 200ng of topoisomerase I-activated pBAD/Thio-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. The second box contains all of the reagents necessary for transformation including 21 single-use 50μL aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid.
- pBAD/Thio-TOPO vector encodes His-Patch thioredoxin as N-terminal fusion partner
- Thioredoxin fusion can significantly increase solubility of many difficult-to-express proteins and improve yield of protein production
- Vector features araBAD promoter for tightly regulated expression in E. coli I-activated vector for 5-minute TOPO Cloning of Taq-amplified PCR products
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with aAnti-His(C-term) Antibody
- C-terminal V5 epitope for detection with an Anti-V5 Antibody
- Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMaxEnterokinase
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications
Specifications
Constitutive or Inducible System | Inducible |
Inducing Agent | Arabinose |
Promoter | araBAD |
Product Type | Lentiviral Expression Kit |
Selection Agent (Eukaryotic) | None |
Content And Storage | Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (except Taq polymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
Bacterial or Yeast Strain | TOP10 |
Cleavage | EK (Enterokinase) Recognition Site |
Expression System | E. coli |
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For Research Use Only. Not for use in diagnostic procedures.
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