Invitrogen™ pBAD202 Directional TOPO™ Expression Kit

Description

Clones PCR product at high efficiency in five minutes into a topoisomerase I-activated expression vector

Using restriction enzymes to clone gene into an expression vector often forces to compromise final sequence of insert, especially when there are no useful restriction sites close to genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce expression levels and/or cause production of non-functional protein.

• TOPO Expression Vectors enable to insert exact DNA sequence require simply by performing PCR with appropriately designed primers
• Resulting recombinant expression vector contains exact DNA sequence without any noncoding regions
• Several expression vectors are adapted for Directional TOPO Cloning, enabling to use proofreading polymerases and to clone the PCR products in a specific orientation

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Inducing Agent: Arabinose
• Promoter: araBAD
• Product Type: TOPO Expression Kit
• Selection Agent (Eukaryotic): None
• Antibiotic Resistance Bacterial: Kanamycin (KanR)
• Bacterial or Yeast Strain: LMG194, TOP10
• Cleavage: EK (Enterokinase) Recognition Site
• Expression System: E. coli
• Expression Mechanism: Cell-Based Expression
• Protein Tag: His Tag (6x), V5 Epitope Tag, Thioredoxin
• Cloning Method: Directional TOPO
• Quantity: 20 reactions
• Vector: pBAD
• Product Line: TOPO™

For Research Use Only. Not for use in diagnostic procedures.