Invitrogen™ pBudCE4.1 Mammalian Expression Vector

Description

Includes

20μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized.

Designed for the independent expression of two genes from a single plasmid in mammalian cells

• Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in cell; this can eliminate variable expression due to differences in gene copy number
• Provides expression cassettes with the CMV promoter for high-level transcription of genes with optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
• Human EF-1α promoter for high-level expression of genes with optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
• Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coli with selection agent Zeocin™

Constitutive Expression, Mammalian Expression, Multi-Gene Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: EF-1α, CMV
• Product Type: Mammalian Expression Vector
• Selection Agent (Eukaryotic): Zeocin™
• Content And Storage: 20 μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x), V5 Epitope Tag, c-Myc Epitope Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pBud
• For Use With (Application): Multi-Gene Expression

For Research Use Only. Not for use in diagnostic procedures.