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  5. Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit 

Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Catalog No. K490040
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Description

Includes

Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

  • Proofreading enzyme is used: fewer errors in cloned genes
  • Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
  • CMV promoter: high expression levels
  • C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
  • 6xHis tag for rapid protein purification on nickel-chelating resin

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type TOPO Expression Kit
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage 2 × Box 1 (Store at -20°C):
• 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl)
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 20 μl T7 Sequencing Primer (0.1 μg/μl)
• 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl)
• 10 μl Control PCR Template (0.1 μg/μl)
• 10 μl Control PCR Primers (0.1 μg/μl each)
• 10 μl Expression Plasmid (0.5 μg/μl)

2 × Box 2 (Store at -80°C):
• 6 ml S.O.C. Medium (may be stored at 4°C or room temperature)
• 21 × 50 μl TOP10 Cells
• 50 μl pUC19 Control DNA
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Directional TOPO
Quantity 40 Reactions
Vector Directional TOPO Vectors, pcDNA
Product Line TOPO, pcDNA
For Use With (Application) Constitutive Expression
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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