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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Description
Includes
Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA
- Proofreading enzyme is used: fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
- CMV promoter: high expression levels
- C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
- 6xHis tag for rapid protein purification on nickel-chelating resin
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | TOPO Expression Kit |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Content And Storage | 2 × Box 1 (Store at -20°C): • 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl) • 10 μl dNTP Mix • 50 μl Salt Solution • 1 ml Sterile Water • 20 μl T7 Sequencing Primer (0.1 μg/μl) • 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl) • 10 μl Control PCR Template (0.1 μg/μl) • 10 μl Control PCR Primers (0.1 μg/μl each) • 10 μl Expression Plasmid (0.5 μg/μl) 2 × Box 2 (Store at -80°C): • 6 ml S.O.C. Medium (may be stored at 4°C or room temperature) • 21 × 50 μl TOP10 Cells • 50 μl pUC19 Control DNA |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Directional TOPO |
| Quantity | 40 Reactions |
| Vector | Directional TOPO Vectors, pcDNA |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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