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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression
$1172.00 - $2105.00
Specifications
Constitutive or Inducible System | Constitutive |
---|---|
Delivery Type | Transfection |
Promoter | CMV |
Product Type | TOPO Expression Kit |
Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
Includes: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
K490001
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Invitrogen™
K490001 |
20 Reactions |
Each for $1,172.00
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K490040
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Invitrogen™
K490040 |
40 Reactions |
Each for $2,105.00
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Description
- Proofreading enzyme is used: fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
- CMV promoter: high expression levels
- C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
- 6xHis tag for rapid protein purification on nickel-chelating resin
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
Constitutive | |
CMV | |
Geneticin™ (G-418) | |
Directional TOPO | |
TOPO™, pcDNA™ |
Transfection | |
TOPO Expression Kit | |
His Tag (6x), V5 Epitope Tag | |
Directional TOPO Vectors, pcDNA | |
Constitutive Expression |
For Research Use Only. Not for use in diagnostic procedures.
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