Invitrogen™ pcDNA™3.1 ^(-) Mammalian Expression Vector

Description

Includes

20μg of this pcDNA3.1^(-) vector, as well as an expression control, are supplied supercoiled and lyophilized.

Designed for high-level, constitutive expression in a variety of mammalian cell lines

• Contains Geneticin selectable marker and forward-orientation multiple cloning site
• For use alone or in co-transfections
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in reverse (-) orientation
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing large T antigen (i.e.,COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Mammalian Expression Vector
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: 20 μg of this pcDNA™3.1^(-) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
• Protein Tag: Untagged
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pcDNA
• Product Line: pcDNA™
• For Use With (Application): Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.