Invitrogen™ pcDNA™3.3-TOPO™ TA Cloning™ Kit

Description

Includes

• Box 1: 20μL pcDNA3.3-TOPO vector, TOPO adapted (5 to 10ng/μL), 100μL 10X PCR Buffer, 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 10μL Control PCR Template (50ng/μL), 10μL Control PCR Primers (100ng/μL each), 20μL CMV Forward Sequencing Primer (100ng/μL), 20μL TK polyA Reverse Sequencing Primer (100ng/μL, 10μL pcDNA3.3-TOPO/lacZ Expression Control Plasmid (500ng/μL)
• Box 2: 11 x 50μL TOP10 E. coli; 50μL pUC19 Control DNA (10pg/μL), 6mL S.O.C. Medium

Designed to deliver exceptionally high levels of transgene expression in adherent cells

• Supplied with TOPO-adapted plasmid vector, all reagents for cloning and One Shot TOP10 competent cells
• pcDNA3.3-TOPO vector offers TOPO adapted for cloning PCR products with >85% efficiency
• High-level gene expression in wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp)
• Vector is excellent for large-scale protein expression, generation of native protein without extraneous amino acids

Antibody and Cell Culture Production, Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Dry Ice

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: TOPO TA Cloning Kit
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: Box 1 (store at -20°C)
  • 20 μl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/μl)
  • 100 μl 10X PCR Buffer
  • 10 μl dNTP Mix
  • 50 μl Salt Solution
  • 1 ml Sterile Water
  • 10 μl Control PCR Template (50 ng/μl)
  • 10 μl Control PCR Primers (100 ng/μl each)
  • 20 μl CMV Forward Sequencing Primer (100 ng/μl)
  • 20 μl TK polyA Reverse Sequencing Primer (100 ng/μl)
  • 10 μl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/μl)
  
  Box 2 (store at -80°C)
  • 11 × 50 μl TOP10 E. coli
  • 50 μl pUC19 Control DNA (10 pg/μl)
  • 6 ml S.O.C. Medium
• Protein Tag: Untagged
• Cloning Method: TOPO-TA
• Quantity: 20 reactions
• Vector: pcDNA
• Product Line: TOPO™, pcDNA™
• For Use With (Application): Protein Production, Constitutive Expression

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.