Invitrogen™ pcDNA™4/HisMax A, B, & C Mammalian Expression Vectors

Description

Includes

The pcDNA4/HisMax A, B and C vectors, 20μg each, are supercoiled and provided lyophilized. 20μg of pcDNA4/HisMax/lacZ control plasmid is also provided. The pcDNA4/HisMax TOPO TA Expression Kit contains two boxes. The TOPO TA box contains 200ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid. The box contains transformation reagents including 21 x 50μL aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid.

Designed to maximize protein expression in a variety of mammalian cells

• Contains QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with promoter alone
• In addition to SP163-enhanced expression, pcDNA4/HisMax includes cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with Anti-Xpress Antibody

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Mammalian Expression Vector
• Selection Agent (Eukaryotic): Zeocin™
• Content And Storage:

  The pcDNA™4/HisMax A, B, & C vectors, 20 μg each, are supercoiled and provided lyophilized. 20 μg of pcDNA™4/HisMax/lacZ control plasmid is also provided. Store vectors at -20°C. The pcDNA™4/HisMax TOPO™TA Expression Kit contains two boxes. The TOPO™ TA box contains 200 ng of linearized and topoisomerase I-activated pcDNA4/HisMax-TOPO™ vector, dNTPs, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a lacZ expression control plasmid.
  Store this box at -20°C. The One Shot™ box contains transformation reagents including twenty-one 50-μl aliquots of TOP10 E. coli, S.O.C. medium, and a pUC19 supercoiled control plasmid. The One Shot™ box should be stored at -80°C. Guaranteed stable for 6 months when properly stored.

• Cleavage: EK (Enterokinase) Recognition Site
• Protein Tag: His Tag (6x), Xpress Epitope Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pcDNA
• Product Line: pcDNA™
• For Use With (Application): Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.