Invitrogen™ pDisplay™ Mammalian Expression Vector

Description

Includes

20μg pDisplay vector is supplied supercoiled and lyophilized.

Designed to target recombinant proteins to the surface of mammalian cells

• Displayed proteins can be analyzed for their ability to interact with known or putative ligands
• Proteins of interest are targeted and anchored to cell surface by cloning the gene of interest in frame with vectors unique N-terminal secretion signal and C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR)
• In contrast to phage display vectors which operate exclusively in prokaryotic cells, pDisplay vector offers advantage of having displayed protein of interest processed in mammalian cells
• Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay more closely resemble their native form: this may favor more accurate ligand binding interactions
• In addition to N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, pDisplay vector contains T7 promoter/priming site for in vitro transcription of sense RNA and for sequencing of inserts
• Neomycin resistance marker for stable selection in mammalian cells using Geneticin™
• SV40 origin for replication and simple vector rescue in cell lines expressing large T antigen (e.g.,COS-1 and COS-7)
• Ampicillin resistance gene for selection in E. coli

Mammalian Two-Hybrid, Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis

Specifications

• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Mammalian Expression Vector
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: 20 μg pDisplay vector is supercoiled and supplied as a 0.5 μg/mL solution in TE buffer, pH 8.0. Store at -20°C. Guaranteed stable for 6 months when properly stored.
• Protein Tag: IgK Leader Sequence, PDGFR Transmembrane Domain, c-Myc Epitope Tag, HA Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pDisplay
• Product Line: pDISPLAY™

For Research Use Only. Not for use in diagnostic procedures.