Invitrogen™ pGlow TOPO™ TA Expression Kit

Description

Includes

pGlow TOPO TA Expression Kits contains two boxes. The TOPO TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers. The box contains TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid.

Uses Cycle 3 GFP mutant as reporter protein for transcriptional activity

• Cycle 3 GFP is exellent for noninvasive analysis of promoter elements within intact, living cells
Fluorescent property of Cycle 3 GFP allows
• in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

• Delivery Type: Transfection
• Promoter: None (Promoterless)
• Product Type: TOPO TA Expression Kit
• Reporter Gene: GFP (Cycle 3)
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.
• Protein Tag: Untagged
• Cloning Method: TOPO-TA
• Quantity: 20 reactions
• Vector: pGlow, TOPO-TA Vectors
• Product Line: TOPO™
• For Use With (Application): Reporter Assays, Clone your own Promoter

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.