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Invitrogen™ pIB/V5-His TOPO™ TA Expression Kit
Description
Includes
Each pIB/V5-His TOPO TA Expression Kit contains two boxes. The pIB/V5-His TOPO box contains 200ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. The box contains high-efficiency, single-use, 50μL aliquots of chemically competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control.
- The OpIE2 promoter for constitutive expression
- Includes Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification using nickel-chelating resin
Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | OplE2 |
| Product Type | TOPO TA Expression Kit |
| Content And Storage | Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. Store at -20°C. The One Shot™ box contains high-efficiency, single-use, 50 μl aliquots of chemically competent TOP10 E. coli cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag Position (to your gene) | C-terminal |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | TOPO-TA |
| Quantity | 20 reactions |
| Vector | pIB, TOPO-TA Cloning Vectors |
| Product Line | InsectSelect™, TOPO™ |
For Research Use Only. Not for use in diagnostic procedures.
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