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Invitrogen™ pIZ/V5-His Vector Kit
Description
Includes
The InsectSelect Kit includes 20μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2μg of lyophilized OpIE2 Reverse Sequencing primer, 1g Zeocin, Cellfectin Reagent, and 1L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO Grace's Insect Media, High Five Cells are supplied with GIBCO Express Five Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above.
- This exceptionally small vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells
- The OpIE2 promoter for constitutive expression
- The Zeocin resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resin
Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | OplE2 |
| Product Type | Insect Cell Expression Vector |
| Content And Storage | The InsectSelect™ Kit includes 20 μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 μg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin™ Reagent, and 1 L of medium for the insect cell line chosen. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with GIBCO™ Express Five™ Serum-Free Medium. The pIZ/V5-His Vector Kit contains only the expression vectors and primer as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag Position (to your gene) | C-terminal |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 1 kit |
| Vector | pIZ |
| Product Line | InsectSelect™ |
For Research Use Only. Not for use in diagnostic procedures.
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