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Invitrogen™ pIZT/V5-His Vector Kit
Description
Includes
The InsectSelect Glow Kit includes 20μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1g Zeocin, Cellfectin Reagent, Sf9 cells, and 1 L of GIBCO Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above.
- The OpIE2 promoter for constitutive expression
- The Zeocin resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin
- In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP)
- The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells
Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | OplE2 |
| Product Type | Insect Cell Expression Vector |
| Content And Storage | The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag Position (to your gene) | C-terminal |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 1 kit |
| Vector | pIZ |
| Product Line | InsectSelect™ |
For Research Use Only. Not for use in diagnostic procedures.
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