Invitrogen™ Plasmid pCMV·SPORT-βgal

Catalog No.	Quantity
10-586-014	25 μg

Catalog No. 10-586-014 Supplier Invitrogen™ Supplier No. 10586014

Description

Used as a positive control for monitoring expression in eukaryotic cells

• Plasmid contains reporter gene b-galactosidase (b-gal) from E. coli cloned as Not I fragment into plasmid pCMV·SPORT1
• Plasmid also contains CMV promoter upstream of b-gal gene, followed by the SV40 t-intron and polyadenylation signal
• b-lactamase gene allows selection for ampicillin resistance in E. coli

Antibody and Cell Culture Production, Beta-Galactosidase Assays, Bioproduction, Constitutive Expression/Enzyme and Protein Activity Assays, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Plasmid
• Reporter Gene: Beta-Gal (lacZ)
• Selection Agent (Eukaryotic): None
• Content And Storage: Store at +4°C. Store at -20°C long term.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Protein Tag: Untagged
• Quantity: 25 μg
• Vector: pCMV
• For Use With (Application): Reporter Assays, Constitutive Expression

Frequently Asked Questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.