Invitrogen™ pMIB/V5-His Vector Kit

Description

Includes

20μg each of supercoiled, lyophilized pMIB/V5-His A, B and C vectors, pMIB/V5-His/CAT control vector, and 2μg each of the OpIE2 Forward and Reverse Sequencing primers.

3.6kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five insect cells

• Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of the protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Anti-V5 antibody and purification with nickel-chelating resin

Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells

Specifications

• Promoter: OplE2
• Product Type: Insect Cell Expression Vector
• Content And Storage: The pMIB/V5-His Vector Kit contains 20 μg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 μg each of the OpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
• Protein Tag Position (to your gene): C-terminal
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pMIB
• Product Line: InsectSelect™

For Research Use Only. Not for use in diagnostic procedures.