Invitrogen™ pOG44 Flp-Recombinase Expression Vector

Description

Includes

The TOPO Cloning box contains all of the reagents necessary to clone a Taq-amplified PCR product (TOPO TA) or a PCR product generated with a proofreading enzyme (Directional TOPO), including 200ng of topoisomerase I-activated vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, primers for sequencing, and an expression control plasmid. The Competent Cell box contains all of the reagents necessary for transformation, including 21 x 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. The pEF5/FRT/V5-DEST vector contains 6μg of lyophilized vector and a control plasmid.

For efficient integration into Flp-In cell lines

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: Flp-In System Expression Vector
• Selection Agent (Eukaryotic): None
• Content And Storage: The pcDNA™5/FRT/V5-His TOPO™ TA Expression Kit, pSecTag/FRT/V5-His TOPO™ TA Expression Kit, and the pEF5/FRT/V5 Directional TOPO™ Expression Kit contain two boxes. The TOPO™ Cloning box contains all of the reagents necessary to clone a Taq-amplified PCR product (TOPO™ TA) or a PCR product generated with a proofreading enzyme (Directional TOPO™), including 200 ng of topoisomerase Iactivated vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. The pEF5/FRT/V5-DEST™ vector contains 6 μg of lyophilized vector and a control plasmid. Store at -20°C once resuspended. All reagents are guaranteed stable for 6 months when properly stored.
• Protein Tag: Untagged
• Cloning Method: TOPO, Gateway
• Quantity: 20 μg
• Vector: pOG44
• Product Line: Flp-In™
• For Use With (Application): Targeted Integration

For Research Use Only. Not for use in diagnostic procedures.