Invitrogen™ pPICZ A, B, & C Pichia Vectors

Description

Includes

The EasySelect PichiaExpression Kit includes 20μg each of pPICZ A, B, and C and pPICZ A, B, and C, Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression, 250mg Zeocin , media (YP and YNB), EasyComp PichiaTransformation Kit, 2μg each 5' AOX1, 3' AOX1,and α-factor sequencing primers. A lacZ positive expression control is provided with pPICZ.

Designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein

• These vectors contain Zeocin™ resistance gene for direct selection of multi-copy integrant strains
• By selecting with increasing amounts of Zeocin, strains with multiple copies of the gene of interest integrated into genome are obtained
• Increasing number of copies of gene of interest in recombinant Pichiastrain can result in higher expression levels
• Inducible AOX1promoter for high-level expression in Pichia pastoris c-mycepitope tag for convenient detection with Anti-mycAntibody
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody
• The pPICZ vector also features secretion signal for efficient transport of proteins to medium

Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression

Specifications

• Promoter: AOX1
• Product Type: Pichia Expression Vector
• Content And Storage: 20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.
  
  Store vectors at -5 to -30°C.
• Antibiotic Resistance Bacterial: Zeocin™ (ZeoR)
• Protein Tag: His Tag (6x), c-Myc Epitope Tag
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 20 μg
• Vector: pPIC

For Research Use Only. Not for use in diagnostic procedures.