Invitrogen™ Human IL-12 p40 ProQuantum Immunoassay Kit

Description

ELISA

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Specifications

• Accession Number: P29460
• Assay Range: 0.064 to 10000 pg/mL
• Assay Sensitivity: <0.05 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Cytokine Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 3593
• Gene Symbol: CLMF; CLMF2; IL-12 B; IMD28; IMD29; NKSF; NKSF2
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: -20°C
• Target: IL-12 p40
• Test Time: 30 mins
• Total Assay Time: 2 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov